Target of this antibody:
Alternative names of antibody target:
TCTN1 belongs to the Tectonic protein family that regulate Hedgehog (Hh) pathway. During neural tube development, TCTN1 is required for the formation of most ventral cell types and for full Hh pathway activation. At later stages in development, TCTN1 is thoμght to also act as a repressor on the Hh pathway in the anterior and posterior neural tube.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic peptide surrounding amino acid 520 of rat TCTN1
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit anti- TCTN1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western Blot analysis (0.5-4 µg/ml). It detects 64 kDa TCTN1 and its cleavage fragment. However, the optimal concentrations should be determined individually. Blocking peptide (Cat.# 3087BP-50) is available separately.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.