Target of this antibody:
Alternative names of antibody target:
MSF , FINC , DKFZp686F10164 , DKFZp686H0342 , FN 1 , FN , CIG
Fibronectin is a glycoprotein synthesized in the liver for the circulating blood plasma form, and is synthesized by many mesenchymal cells, for the extracellular matrix form. It is composed of two similar, but not identical protein chains, which are linked by two disulfide linkages at the C-terminal end of the chains. The chains are composed of domains which have specific secondary structures linked together by regions which are especially susceptible to proteolytic action. For this reason, detection by immunoblot (western) may show considerable variability in the observed apparent molecular weights, which is predicated on the source of the fibronectin, and to what degree proteolysis has occurred. Bands approximately 225 kDa should be observed after SDS-PAGE when reducing and denaturing conditions are used
Clonality of antibody:
This antibody reacts with:
Purified fibronectin from human plasma
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5mg/ml) antigen affinity purified rabbit anti-Fibronectin polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 0.1% BSA and 0.02% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blot, Immunohistochemistry
Western blotting (0.5 – 2.0 µg/ml), Immunohistochemistry (20-40 µg/ml). However, the optimal concentrations should be determined individually.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.