Target of this antibody:
Alternative names of antibody target:
CRP55, CRTC, CALR, Calregulin, cC1qR, ERp60, HACBP, RO, SSA,
Calreticulin is a calcium binding protein found in abundance in the endoplasmic reticulum and the sarcoplasmic reticulum. Like many other ER proteins, it has the conserved ER retention KDEL (Lys-Asp-Glu-Leu) sequence at its C-terminus. Calreticulin has also been detected in the nucleus and nuclear envelop. Recent studies sμggest that this soluble ER protein has a multifunctional role as it appears to be involved in calcium storage and regulation as well as having a molecular chaperone activity. Studies also sμggest its involvement in certain autoimmune diseases. Consistence with its multiple functions the calreticulin molecule appears to have a zonal character. The protein has both high and low affinity calcium binding sites.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic peptide mapping to the C-terminus of Human Calreticulin
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (200 µg/ml) protein A purified antibody in PBS containing 30% glycerol, 0.5 mg/ml BSA, and 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blot, Immunohistochemistry
Western blot analysis (0.5-4 µg/ml) and in Immunohistochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.