Target of this antibody:
Alternative names of antibody target:
TP53, TRP53, P53, LFS1, Tumor suppressor p53, Phosphoprotein p53
p53, a 53 kDa protein, binds to a DNA consensus sequence, the p53 response element, and regulates normal cell cycle events by activating transcription of genes involved either in progression throμgh the cell cycle, or causing arrest in G1 when the genome is damaged. In most transformed and tumor cells the concentration of p53 is increased 5-1000 fold over the concentration in normal cells, principally due to the increased half-life (4 hrs) compared to that of wild-type (20 min). p53 localizes in the nucleus, but is detectable at the plasma membrane during mitosis and certain mutations also modulate cytoplasmic/nuclear distribution. p53 downregulates Bcl-2 expression and upregulates Bax expression, but may not always be necessary for apoptosis.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat, dog, rabbit
Synthetic peptide corresponding to residue surrounding amino acid 375 of human p53
Concentration of antibody:
Form of antibody:
This antibody has been succesfully tested for use in following methods:
Western blot analysis (0.5-4 µg/ml). It detects p53 in samples of human, mouse and rat origins. Other applications have not been determined.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.