Target of this antibody:
Alternative names of antibody target:
Vasodilator-stimulated phosphoprotein (VASP) belongs to the Ena/VASP family of adaptor proteins linking the cytoskeletal system to the signal transduction pathways. VASP functions in cytoskeletal organization, fibroblast migration, platelet activation and axon guidance. Three phosphorylation sites, Ser157, Ser239 and Thr278, have been identified. Ser157 is the major PKA phosphorylation site. Evidence sμggests that VASP phosphorylation reduces its association with actin and has a negative effect on actin polymerization.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic phospho peptide surrounding amino acid Ser157 of human VASP
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in 1X phosphate-buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blotting (0.5-4 µg/ml) and Immunohistochemistry. However, the optimal concentrations should be determined individually. The antibody recognizes 51 kDa phosphorylated VASP from samples of human, mouse, and rat origins. Reactivity to other species has not been tested.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.