Target of this antibody:
Alternative names of antibody target:
PS1TP5BP1 , ACTB, Beta-actin , b-actin
Actin is expressed in all eukaryotic cells and is the major component of the cytoskeleton. At least six types of actin are present in mammalian tissues and fall into three classes. Alpha actin expression is limited to various types of muscle and it regulates contactile potentials for the muscle cells, whereas beta and gamma actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic peptide corresponding to residues around amino acid 10 of human Actin
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
30 µg (0.2mg/ml) protein A purified rabbit anti-β-Actin polyclonal antibody in phosphate-buffered saline (PBS) containing 0.5 % BSA, 30% glycerol, and 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blotting (0.5-2 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes 45 kDa beta-Actin in samples from human and mouse origins. Reactivity to other species has not been tested.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.