Target of this antibody:
Alternative names of antibody target:
CAMK2A ,CAMKA ,KIAA0968,EC 220.127.116.11
CaMKII is a member of calcium/calmodulin-activated kinase, functioning in neural synaptic stimulation and T-cell receptor signaling. CaMKII has catalytic and regulatory domains. The binding of Ca++/calmodulin to its regulatory domain releases its autoinhibitory effect and activates the kinase. The activated CaMKII further autophosphorylates itself at Thr286 to render the kinase constitutively active.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic phospho-peptide corresponding to residues surrounding Thr286 of human CaMKII
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µl purified rabbit polyclonal anti-Phospho-CaMKII (Thr286) antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blotting at 1-2 µg/ml. However, the optimal concentrations should be determined individually. The anti-Phospho-CaMKII antibody recognizes only the phosphorylated CaMKII (Thr286) in samples from human, mouse, and rat origins.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.