Target of this antibody:
Alternative names of antibody target:
USP7, ubiquitin specific processing protease 7; HAUSP, Herpes virus-associated ubiquitin-specific protease; Deubiquitinating enzyme 7; Ubiquitin thiolesterase 7; Ubiquitin carboxyl-terminal hydrolase 7; TEF1
Ubiquitin (Ub) is a highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific processing protease 7 (USP7) is a member of this family that has been shown to interact and stabilize p53.
Clonality of antibody:
This antibody reacts with:
Human, mouse, chicken
Synthetic peptide of human USP7
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µl affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blotting (1:500 – 1:2000). However, the optimal concentrations should be determined individually. The antibody recognizes USP7 of human origin. HeLa cell nuclear extract (Cat.# 1641-1) can be used as a positive control. Based on sequence homology, the antibody should also react with mouse and chick samples.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.