Target of this antibody:
Alternative names of antibody target:
RASGAP, p120GAP , RASA1, RASA, CMAVM, CM-AVM , PKWS , p120GAP, DKFZp434N071
The mammalian proto-oncogene Ras encodes 21 kDa proteins (p21Ras) which exists in either a physiologically quiescent GDP-binding state or a GTPbinding signal-emitting state. Interaction of p21Ras with GTPase activating protein (GAP) can increase hydrolysis of p21Ras-bound GTP by as much as 1000-fold. The product of the neurofibromatosis type 1 gene (NF1) has also been shown to exhibit p21Ras GAP activity.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat, bovine
Synthetic peptide surrounding amino acid 1025 of human RasGAP
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.2 mg/ml) protein A purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blot, Immunoprecipitation, Immunohistochemistry
Western blotting (0.5-4 µg/ml) and in immunoprecipitation (10-20 µg/ml) and Immunohistochemistry (10-20 µg/ml). However, the optimal concentrations should be determined individually. Jurkat cell lysate can be used as a positive control. The antibody recognizes ~120 kDa Ras GAP of human, mouse, rat, and bovine origins.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.