Target of this antibody:
Alternative names of antibody target:
MAP3K5, MEKK5, ASK-1 , MAPKKK5
ASK1 (Apoptosis Signal-regulating Kinase 1) activates two different subgroups of MAPKK, MKK4 and MKK6, which in turn activate c-Jun N-terminal Kinase (JNK) and p38 MAP Kinase. ASK1 is activated by TNFR and Fas throμgh the interaction with members of the TRAF Family and the Fas-associated protein Daxx. Overexpression of ASK1 induces apoptosis. However, a catalytically inactive form of ASK1 has been shown to inhibit TNF-α induced apoptosis.
Clonality of antibody:
This antibody reacts with:
Synthetic peptide corresponding to residue surrounding amino acid 1359 of human ASK1
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.2 mg/ml) immunoaffinity purified, rabbit anti-ASK1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% sodium azide.
This antibody has been succesfully tested for use in following methods:
Western blot, Immunofluorescence
Western blot analysis (1-4 µg/ml). However, the optimal conditions should be determined individually. The immunoaffinity purified antibody detects the 155 kDa ASK1 protein in human SW1353 cell lysate by immunoblotting.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.