Target of this antibody:
Alternative names of antibody target:
581, BAX, 600040, P55269, Bax zeta
The Bcl-2 (B-cell leukemia/lymphoma 2) family proteins function to either inhibit (Bcl-2 and Bcl-XL) or promote (Bax and Bak) apoptosis. Bax contains the conserved Bcl-2 homology 1 (BH1) and 2 (BH2) domains that allow for its homodimerization or heterodimerization with Bcl-2. When Bcl-2 is in excess, apoptosis is inhibited. However, if Bax levels increase in response to a death signal, the cell is pushed toward death.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat, monkey
Synthetic peptide corresponding to amino acids near the N-terminus of human Bax
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit anti-Bax polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blot, Immunoprecipitation
Western blot analysis (0.5-4 µg/ml), immunoprecipitation (10-20 µg/ml). However, the optimal conditions should be determined individually.The antibody detects a 20 kDa protein, corresponding to the apparent molecular weight of Bax on SDS-PAGE immunoblots.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.