Target of this antibody:
Alternative names of antibody target:
CFLAR , I-FLICE, CASP8AP1, Casper, Usurpin, c-FLIPS , FLAME , CASH , c-FLIPL, CLARP, c-FLIPR, c-FLIP , MRIT , FLAME1
c-FLIP was cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH AND CLARP. c-FLIP contains two death effector domains and has sequence homology to caspase-8. c-FLIP interacts with adapter protein FADD and caspaser-8 and -10, and potently inhibits apoptosis induced by all known death receptors. Two splice variants of c-FLIP have been identified and termed FLIPl and FLIPs
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic peptide near the C-terminus of human FLIPa/FLIPL form
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.2 mg/ml) affinity purified rabbit anti-FLIP polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blot, Immunofluorescence
Western blot analysis (0.5-4 µg/ml) and immunofluorescence. However, the optimal conditions should be determined individually. The immunoaffinity-purified antibody detects 58 kDa FLIPL on SDS-PAGE immunoblots, in samples from human, mouse and rat origins. The antibody recognizes only the FLIPL form.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.