EZAct™ Procathepsin Activation Buffer

Catalog number
EZAct™ Procathepsin Activation Buffer
1 ml
153.00 EUR


APPLICATIONS: 1. To generate active Cathepsins from inactive Procathepsins. 2. To test the activity of Cathepsins. 3. To screen for Cathepsin Inhibitor candidates. ACTIVATION PROTOCOL: 1. Adjust the concentration of the target Procathepsin to 0.5-5 mg/ml with the appropriate buffer. 2. Add equal amounts of EZAct™ Procathepsin Activation Buffer and mix well by pipetting up and down. 3. Incubate at the appropriate temperature (4°/ 22°/ 37°C) for the study. 4. Check the cathepsin activation by testing with an appropriate substrate. Note: BioVision’s Cathepsin Assay kits can be used for assaying the Cathepsin activity. Choose from assay kits: K140, K141, K142, K143, K144, K145 and K146.
Cathepsins (CTS) are proteases that degrade proteins. They are present in all animals and other organisms. These proteases are of three types: cysteine proteases, aspartyl protease and serine proteases. Cathepsins are produced as inactive proenzyme precursors that are subsequently activated in lysosomes. Biovision’s EZAct™ Procathepsin Activation Buffer is a ready-to-use buffer for generation of active cysteine/aspartyl Cathepsins by auto-activation of the Procathepsins.
Procathepsin Buffers
Storage condition
Shipping condition
gel pack
Maximum time for storage
12 months
Additional description
Buffering solutions are useful to keep the pH range sable when using this reagent of Biovision.