Target of this antibody:
Alternative names of antibody target:
LIN28 is a highly conserved cytoplasmic RNAbinding protein. Lin28 has an N-terminal cold shock domain and two Cterminal (CCHC) zinc finger motif. It is expressed in embryonic stem cells and embryonic carcinoma cells. Lin28 is also found in adult cardiac and skeletal muscle.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic peptide surrounding amino acid 101 of rat Lin28
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
200 µg (0.5 mg/ml) affinity purified rabbit anti-Lin28 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western Blot analysis (2-4 µg/ml). However, the optimal concentrations should be determined individually. Blocking peptide (Cat.# 3091BP-50) is available separately.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.