Target of this antibody:
Alternative names of antibody target:
PRKDC, XRCC7 , p460, DNPK1, HYRC, HYRC1 , DNAPK ,p350
DNA-dependent protein kinase (DNA-PK) is made up of 3 subunits, 2 Ku proteins and a large catalytic subunit (DNA-PKcs). DNA-PKcs falls into the phosphatidyl-inositol 3-kinase superfamily althoμgh it does have serine/threonine protein kinase activity. This subunit migrates on SDS-PAGE gels as an intact and smaller processed form. In vitro, DNA-PK phosphorylates several transcription factors and other DNA-binding proteins and is believed to play a role in DNA damage recognition, repair and transcription. There is evidence to sμggest that DNA-PKcs may be a critical target for proteolysis by a caspase in apoptosis.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat, hamster
Synthetic peptide corresponding to residues surrounding amino acid 4088 of human DNA-PK
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) immunoaffinity purified rabbit anti-DNA-PK polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The immunoaffinity-purified antibody detects a>350,000 kDa and a 180,000 kDa protein (believed to be a processed form of the catalytic subunit), corresponding to the expected molecular weight of DNA-PKcs, in samples from human, mouse, rat and hamster origins.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.