Target of this antibody:
Alternative names of antibody target:
signal transducer and activator of transcription 1
STATs (signal transducers and activators of transcription) are a family of cytoplamic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signalling peptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STATs enter the nucleus to regulate transcription of many different genes. Among the seven STATs (Stat1, Stat2, Stat3, Stat4, Stat5a, Stat5b, and Stat6). Stat1, Stat3, Stat5a and Stat5b have wide activation profiles. Stat1 is activated by many different ligands including IFN family (IFN-a, IFN-b, IFN-r, and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF-1), and receptor tyrosine kinases (EGF, PDGF, and CSF-1). Stat1 has two forms, the 91 kDa STAT1α and the 84 kDa STAT1β, which are coded by the same gene with splicing variant.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic peptide surrounding amino acid 672 of rat Stat1
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes both alpha (91 kDa) and beta (84 kDa) subunits of STAT1 in samples from human, mouse, and rat origins.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.