Target of this antibody:
Alternative names of antibody target:
Homeobox transcription factor Nanog, Homeobox protein nanog, hNanog
NANOG is a transcription regulator involved with inner cell mass and embryonic stem (ES) cell proliferation and self-renewal. It imposes pluripotency on ES cells and prevents their differentiation towards extra-embryonic endoderm and trophectoderm lineages. This protein blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes.
Clonality of antibody:
This antibody reacts with:
His fusion recombinant protein of human FOXP3
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.25 mg/ml) purified rabbit Ig polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
This antibody has been succesfully tested for use in following methods:
ELISA, Western blot
The antibody can be used for ELISA (0.25 µg/ml) and Western Blotting (2.5 – 5.0 µg/ml).
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.