Target of this antibody:
Alternative names of antibody target:
TALL-1, THANK, BLyS, TNFSF13B, TNFSF20, CD257, DTL, TALL-1, ZTNF4
BAFF (for B cell Activating Factor) is a novel member in the TNF family. BAFF/BLyS was characterized as a B cell stimulator since it induced B cell proliferation and immunoglobulin secretion. Two receptors for BAFF were recently identified and designated TACI and BCMA. BAFF and its receptors are involved in the development of systemic lupus erythaematosus and other B cell associated autoimmune diseases. Like TNF-α and TRAIL, THANK was shown to activate NF-kB and c-Jun N-terminal kinase (JNK) and to induce apoptosis.
Clonality of antibody:
This antibody reacts with:
Recombinant human BAFF
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit anti-BAFF polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. A 32 kDa full length BAFF and the 18 kDa cleavage fragment can be detected.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.