anti-TACE Antibody
Catalog number
3222-100
Name
anti-TACE Antibody
Size
TACE Antibody
Price
338.00 EUR
Supplier
Details
Target of this antibody:
TACE/ADAM17
Alternative names of antibody target:
TACE, CD156b, Disintegrin and metalloproteinase domain-containing protein 17; ADAM 17; TNF-alpha-converting enzyme; TNF-alpha convertase; Snake venom-like protease
Background information:
Tumor necrosis factor-α (TNF-α) is a proinflammatory cytokine and contributes to a variety of inflammatory disease responses and programmed cell death. TNF-α is synthesized as a 26K type II membrane-bound precursor that is cleaved by a convertase to generate secreted 17K mature TNF-α. TNF-α converting enzyme (TACE) protein was recently purified and the human and mouse TACE cDNAs were cloned by several groups separately. TACE is a membrane-bond metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease). TACE also processes other cell surface proteins, including TNF receptor, TGF-α, L-selectin adhesion molecule, and alpha-cleavage of amyloid protein precursor (APP). TACE mRNA is expressed in a variety of human and murine tissues.
Antibody host:
Rabbit
Clonality of antibody:
Polyclonal
Isotype:
Rabbit IgG
This antibody reacts with:
Human, mouse, rat
Immunogene sequence:
Synthetic peptide surrounding amino acid 810 of human TACE
Concentration of antibody:
0.5 mg/ml
Form of antibody:
Liquid
Formulation of this antibody:
100 µg (0.5 mg/ml) peptide affinity purified rabbit anti-TACE polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.
Purification method:
Affinity purified
This antibody has been succesfully tested for use in following methods:
Western blot
Recommended usage:
Western blotting (1-2 µg/ml). However, the optimal conditions should be determined individually. The immunoaffinity purified antibody detects 80-130 kDa bands, which may represent mature protein, precursor and glycosylated TACE. HeLa and Jurkat cell lysate can be used as positive control.
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions:
gel pack
How to handle this antibody:
The antibody solution should be gently mixed before use.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation
anticorps