Target of this antibody:
Alternative names of antibody target:
MSK1, MSPK1, MGC1911 , RLPK , RSKL
MSK1, a Mitogen and Stress activated protein Kinase, is activated by ERK as well as p38 MAPK in response to growth factors and various cellular stress stimuli. MSK1 resembles RSK by having two kinase domains connected by a regulator linker region. Phosphorylation of RSK1 at Ser 364, Ser381 and Thr574 is critical for RSK1 activity. These sites are analogous to Ser360, Ser376 and Thr581 of MSK1 and maybe important for MSK1 activity as well.
Clonality of antibody:
This antibody reacts with:
Synthetic peptide surrounding phospho-Thr581 of human MSK1
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit anti-Phospho-MSK1(Thr581) polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blot (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Phospho-MSK1(Thr581) detects 90 kDa MSK1 only when phosphorylated at Thr581. The antibody does not cross-react with nonphosphorylated MSK1 or MSK1 phosphorylated at other sites. It does not cross-react with RSK1, RSK2 or RSK3.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.