Target of this antibody:
Alternative names of antibody target:
Profilin I, Pfn1, Epididymis tissue protein Li 184a
Profilin is a protein which maintains and shapes cytoskeleton by binding to actin. Profilin 1 has function in breast cancer cell motility, radial migration and glial cell adhesion.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat, bovine
Synthetic peptide close to C-terminus of rat Profilin-1
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit anti-Profilin-1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
The antibody can be used in Western Blot analysis (1-4 µg/ml). However, the optimal concentrations should be determined individually. Blocking peptide (Cat. # 3306BP-50) is available separately. Expected band is ~15 kDa.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.