Target of this antibody:
Alternative names of antibody target:
PUMA/JFY1 ,JFY-1, PUMA, BBC3, bcl-2 binding component 3
PUMA (p53 upregulated modulator of apoptosis) is one of the pro-apoptotic Bcl-2 family members which are also transcriptional targets of p53. PUMA gene encodes two BH3 domain-containing proteins termed PUMA-α and PUMA-β. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic peptide surrounding amino acid 177 of human PUMA
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit anti-PUMA polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blot, Immunohistochemistry
Western blotting (0.5-4 µg/ml)and Immunohistochemistry (5 µg/ml). However, the optimal conditions should be determined individually. The antibody detects ~25 kDa (193 a.a.) PUMA alpha and ~16 kDa (131 a.a.) PUMA beta in samples from human, mouse and rat origins.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.