Target of this antibody:
Alternative names of antibody target:
Beta-catenin, Catenin beta-1, CTNNB
Catenin is one of the key downstream effectors in the Wnt signaling pathway. It has been implicated to play an important role in early embryonic development and tumorigenesis. β-catenin can be destabilized by GSK-3β or other kinases by phosphorylating it at serines 33, 37, 45 and threonine 41. Mutations of these phosphorylation sites in β-catenin have been found in many tumor cell lines.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic phospho-peptide corresponding to residues surrounding Ser45 of human β-Catenin
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) immunoaffinity purified rabbit polyclonal anti-phospho-β-Catenin antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes 90 kDa phosphorylated β-Catenin (Ser45) of human, mouse, and rat origin.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.