anti-FAK Antibody

Catalog number
3399-100
Name
anti-FAK Antibody
Size
FAK Antibody
Price
338.00 EUR
Supplier

Details

Target of this antibody:
FAK
Alternative names of antibody target:
FAK , FAK1 , FAK-1 , focal adhesion kinase 1 , PTK2, protein tyrosine kinase 2 , FADK1 , pp125FAK
Background information:
Focal adhesion kinase was identified as a 125 kDa substrate for the intrinsic protein tyrosine kinase activity of Src encoded pp60. The deduced amino acid sequence of FAK p125 has shown it to be a cytoplasmic protein tyrosine kinase whose sequence and structural organization are unique as compared to other proteins described to date. Localization of p125 by immunofluorescence sμggests that it is primarily found in cellular focal adhesions leading to its designation as focal adhesion kinase (FAK). FAK is concentrated at the basal edge of only those basal keratinocytes that are actively migrating and rapidly proliferating in repairing burn wounds and is activated and localized to the focal adhesions of spreading keratinocytes in culture. Thus, it has been postulated that FAK may have an important in vivo role in the reepithelialization of human wounds. FAK protein tyrosine kinase activity has also been shown to increase in cells stimulated to grow by use of mitogenic neuropeptides or neurotransmitters acting throμgh G protein coupled receptors.
Antibody host:
Rabbit
Clonality of antibody:
Polyclonal
Isotype:
Rabbit IgG
This antibody reacts with:
Human, mouse, rat
Immunogene sequence:
Recombinant human FAK
Concentration of antibody:
0.5 mg/ml
Form of antibody:
Liquid
Formulation of this antibody:
100 µg (0.5 mg/ml) protein A purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% thimerosal.
Purification method:
Affinity purified
This antibody has been succesfully tested for use in following methods:
Western blot, Immunoprecipitation, Immunofluorescence
Recommended usage:
Western blotting (1-2 µg/ml), immunoprecipitation (5-20 µg/ml), and immunocytochemistry (10-20 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes 125 kDa FAK of human, mouse, and rat origins.
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions:
gel pack
How to handle this antibody:
The antibody solution should be gently mixed before use.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation
anticorps