anti-Phospho-Stat3 Antibody

Catalog number
3474-100
Name
anti-Phospho-Stat3 Antibody
Size
Phospho-Stat3 Antibody
Price
403.00 EUR
Supplier

Details

Target of this antibody:
Phospho-STAT3
Alternative names of antibody target:
MADH3 , JV15-2 , DKFZp686J10186 , hSMAD3 , hMAD-3 , HSPC193 , MGC60396 , HsT17436 , Mad3 , DKFZP586N0721
Background information:
Membrane receptor signaling by various ligands induces activation of Jak kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-α and form a heterodimer which is part of the ISGF3 transcription factor complex. Althoμgh early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3β appears to be activated by both while Stat3α is activated by EGF, but not by IL-6. Highest expression of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 is activated by prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways.
Antibody host:
Rabbit
Clonality of antibody:
Polyclonal
Isotype:
Rabbit IgG
This antibody reacts with:
Human, mouse
Immunogene sequence:
Synthetic phospho-peptide corresponding to resides surrounding Ser727 of mouse Stat3
Concentration of antibody:
0.5 mg/ml
Form of antibody:
Liquid
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% thimerosal.
Purification method:
Affinity purified
This antibody has been succesfully tested for use in following methods:
Western blot, Immunoprecipitation, Immunohistochemistry
Recommended usage:
Western blotting (1-4 µg/ml), immunoprecipitation (20 mg/ml), and Immunohistochemistry (20 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes ~86 kDa phosphorylated Stat3 (Ser727) of human and mouse origins. Reactivity to other species has not been tested.
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions:
gel pack
How to handle this antibody:
The antibody solution should be gently mixed before use.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation
anticorps