anti-Dnmt2 Antibody

Catalog number
3488-100
Name
anti-Dnmt2 Antibody
Size
Dnmt2 Antibody
Price
338.00 EUR
Supplier

Details

Target of this antibody:
DNMT2
Alternative names of antibody target:
Dnmt2 , TRDMT1 , RNMT1 , OTTHUMP00000045198 , cytosine-5-methyltransferase , EC 2.1.1.29
Background information:
Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. Dnmt2 contains all the sequence motifs diagnostic of DNA (cytosine-5)-methyltransferases but appears to lack the large N-terminal regulatory domain common to other eukaryotic methyltransferases.
Antibody host:
Rabbit
Clonality of antibody:
Polyclonal
Isotype:
Rabbit IgG
This antibody reacts with:
Human, mouse
Immunogene sequence:
Synthetic peptides surrounding amino acid 42 and 364 of mouse Dnmt2
Concentration of antibody:
0.5 mg/ml
Form of antibody:
Liquid
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
Purification method:
Affinity purified
This antibody has been succesfully tested for use in following methods:
Western blot, Immunofluorescence
Recommended usage:
Western blotting (0.5-4 µg/ml) and immunofluorescence. However, the optimal concentrations should be determined individually. The antibody recognizes 42 kDa Dnmt 2 of human and mouse origins. Mouse testes tissue lysate can be use as a positive control.
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions:
gel pack
How to handle this antibody:
The antibody solution should be gently mixed before use.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation
anticorps