anti-Phospho-Mek1/2 Antibody

Catalog number
3519-100
Name
anti-Phospho-Mek1/2 Antibody
Size
Phospho-Mek1/2 Antibody
Price
425.00 EUR
Supplier

Details

Target of this antibody:
Phospho-Mek1/2
Alternative names of antibody target:
MAPK , Erk, Dual specificity mitogen-activated protein kinase kinase 1/2; MAP kinase kinase 1/2; MAPKK 1/2; ERK activator kinase 1/2; MAPK/ERK kinase 1/2; MEK 1/2
Background information:
MAP kinase kinase 1 (Mek1), also known as mitogen activated protein kinase kinase, MAP kinase kinase, ERK kinase 1 or microtubule-associated protein 2 kinase, is a 43 kDa dual-specificity protein kinase that phosphorylates threonine and tyrosine residues on MAP kinases such as Erk 1 and 2. Mek1 and Mek2 are involved in a diverse array of cellular processes such as stress-activated response, apoptosis, cytokine-induced cell proliferation, and DNA recombination during meiosis. Mek1 and Mek2 are activated by phosphorylation on serine 218 and 222 by serine-threonine kinase, Raf-1, which is part of the p21ras signal transduction pathway.
Antibody host:
Rabbit
Clonality of antibody:
Polyclonal
Isotype:
Rabbit IgG
This antibody reacts with:
Human, mouse, rat
Immunogene sequence:
Synthetic peptide corresponding to amino acids surrounding Ser217/221 of human MEK1/2
Concentration of antibody:
0.5 mg/ml
Form of antibody:
Liquid
Formulation of this antibody:
100 µl purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% sodium azide.
Purification method:
Affinity purified
This antibody has been succesfully tested for use in following methods:
Western blot, Immunofluorescence
Recommended usage:
Western blotting (1:200-1000) and immunocytochemistry (1:100-200). However, the optimal concentrations should be determined individually. The antibody recognizes 45 kDa phospho-Mek1/2 (Ser217/221). It does not detect the unphosphorylated protein.
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions:
gel pack
How to handle this antibody:
The antibody solution should be gently mixed before use.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation
anticorps