Target of this antibody:
Alternative names of antibody target:
SKD2 , N-ethylmaleimide-sensitive Fusion Protein , Anti-NSF, NSF
Syntaxins were originally thoμght to be docking proteins, but have more recently been categorized as anchoring proteins that anchor themselves to the cytoplasmic surfaces of cellular membranes. Syntaxins have been shown to bind to various proteins involved in exocytosis, including VAMPs (vesicle-associated membrane proteins), NSF (N-ethylmaleimidesensitive factor), SNAP 25 (synaptosomal-associated protein of 25kDa), SNAPs (soluble NSF attachment proteins) and synaptotagmin. SNAPs mediate the membrane binding of NSF, which is essential for membrane fusion reactions. An additional protein designated synaptophysin may regulate exocytosis by competing with SNAP 25 and syntaxins for VAMP binding.
Clonality of antibody:
This antibody reacts with:
Synthetic peptide corresponding to amino acids around 731 of rat NSF
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) Protein A purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% sodium azide.
This antibody has been succesfully tested for use in following methods:
Western blot, Immunoprecipitation, Immunohistochemistry
Western blotting (1-2 µg/ml), immunoprecipitation (5-15 mg/ml) and Immunohistochemistry (10-20 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes 85 kDa NSF of mouse, and rat origins. Reactivity to other species has not been tested
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.