Target of this antibody:
Alternative names of antibody target:
tau , TAU , MAPT , MAPTL , FTDP-17 , MSTD , MGC138549 , PPND , MTBT2
Tau, a microtubule-binding protein which serves to stabilize microtubules in growing axons, is found to be hyperphosphorylated in paired helical filaments (PHF), the major fibrous component of neurofibrillary lesions associated with Alzheimer’s disease. Hyperphosphorylation of Tau is thoμght to be the critical event leading to the assembly of PHF. Six Tau protein isoforms have been identified, all of which are phosphorylated by GSK3. This presents the possibility that miscues in GSK3 signaling contribute to the onset of Alzheimer’s disease.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat, chicken, bovine
Synthetic peptide surrounding amino acid 395 of rat Tau
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit anti-TAU polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Other applications have not been determined. The antibody recognizes mainly the truncated Tau (~30 kDa) in sample from human, mouse and rat origins. Other isoforms of Tau at 35 kDa, 60 kDa and 80 kDa can also be recognized. Reactivity to other species has not been tested.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.