Target of this antibody:
Alternative names of antibody target:
Toll-like receptor 9
The Toll-like receptor (TLR) family of proteins are characterized by a highly conserved Toll homology (TH) domain, which is essential for Toll-induced signal transduction. TLR9 has been sμggested as a receptor for unmethylated CpG dinucleotides in the bacterial DNA. TLR9 is highly expressed in the spleen.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat, pig
Synthetic peptide surrounding amino acid 931 of mouse TLR9
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes ~113 kDa of full length and the 65 kDa cleaved fragment in Jurkat cell lysate. Reactivity to other species has not been tested.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.