Target of this antibody:
Alternative names of antibody target:
Cyclic GMP , Cyclic guanosine monophosphate
Cyclic guanosine monophosphate (cGMP) serves as a second messenger in a manner similar to that observed with cAMP. Peptide hormones, such as the natriuretic factors, activate receptors that are associated with membrane-bound guanylate cyclase (GC). Receptor activation of GC leads to the conversion of GTP to cGMP. Nitric oxide (NO) also stimulates cGMP production by activating soluble GC, perhaps by binding to the heme moiety of the enzyme. Similar to cAMP, cGMP mediates most of its intracellular effects through the activation of specific cGMP dependent protein kinases (PKG).
Clonality of antibody:
This antibody reacts with:
2'O-succinyl guanosine-3',5' cyclic monophosphate
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µl succinylated cGMP affinity purified rabbit IgG in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% thimerosal.
This antibody has been succesfully tested for use in following methods:
1:10-50 dilutions for nonradioactive EIA assays (10 µl/assay in an 70 µl assay reaction). The material is sufficient for performing 100-500 nonradioactive EIA assays or 6,000 radioactive assays. Detect 0.1-10 pmol/assay in a 70 µl assay reaction using nonradioactive method.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.