Target of this antibody:
Alternative names of antibody target:
wnt1, wnt 1, wnt-1, INT 1 , INT1, wingless type MMTV integration site family member 1
Wnt gene family members, including Wnt-1 throμgh Wnt-10, play a key role in regulating cellular growth and differentiation. Wnt-1 is a cysteine-rich, secreted glycoprotein that associates with cell membranes and likely functions as a key regulator of cellular adhesion. Wnt-1, which is essential for normal development of the embryonic nervous system, contributes to hyperplasia and tumorigenic progression when improperly expressed in mammary tissue. Wnt-3 is also involved in tumorigenesis and Wnt-2 and Wnt-4 may be associated with abnormal proliferation in human breast tissue. Wnt-1, Wnt-3 and Wnt-10b have been implicated along with FGF-3 in the development of mouse mammary tumor virus induced mouse mammary carcinomas.
Clonality of antibody:
This antibody reacts with:
Synthetic peptide surrounding amino acid 31 of human Wnt-1
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit Wnt-1 polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blotting (0.5-4 µg/ml), Immunohistochemistry (10-20 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes 41kDa of human Wnt-1. Reactivity to other species has not been tested.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.