anti-KiSS-1 Antibody

Kiss-1

Kisspeptin-1 , kisspeptin , kisspeptin1 , KISS1 , MGC39258

KiSS-1 has been reported to suppress metastases of melanomas and breast carcinomas without affecting tumorigenicity. Human KiSS-1 transcripts are predominately expressed in the brain and placenta. Expression of KiSS-1 reduces the level of NFkB p50/p65 binding to the MMP9 promoter and correlates with diminished expression of the 92 kDa type IV collagenase (MMP-9). KiSS-1 may regulate events downstream of cell-matrix adhesion in mechanisms involving cytoskeletal reorganization.

Rabbit

Polyclonal

Rabbit IgG

Human, mouse, rat

Synthetic peptide surrounding amino acid 17 of mouse KiSS-1

0.5 mg/ml

Liquid

100 µg (0.2 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.

Affinity purified

Western blot

Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. Jurkat cell lysate can be used as a positive control. The antibody recognizes 16 kDa human KiSS-1 in Western blot analysis with Jurkat cell lysate. Blocking peptide (Cat.# 3590BP-50) is available separately.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps