Target of this antibody:
Alternative names of antibody target:
HDAC-2, Histone Deacetylase 2, RPD3, YAF1
HDAC2 is a class I mammalian histone deacetylase containing 488 amino acid residues with an apparent size of ~55 kDa by denaturing SDS-PAGE. HDAC2 has been shown to interact directly with transcription repressor complexes and the nuclear receptor corepressor N-CoR.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat, chicken, hamster, dog
Synthetic peptide surrounding amino acid 479 of human HDAC2
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.2 mg/ml) affinity purified rabbit anti-HDAC2 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blot, Immunocytochemistry
Western blotting (0.5-4 µg/ml) and immunocytochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually. The antibody detects 55 kDa Histone Deacetylase 2 in samples from human, mouse, rat, chicken, hamster, and canine origins.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.