Target of this antibody:
Alternative names of antibody target:
HD5 , FLJ90614 , KIAA0600 , NY-CO-9
Human HDAC5 is composed of 1122 amino acid residues. The deacetylase domain of HDAC5 is located at the C-terminal half of the molecule. The N-terminal non-deacetylase domain does not show any significant homology with any published sequence. Both domains are required for HDAC5-mediated repression of gene transcription. HDAC5 interacts with a growing number of transcriptional factors including MEF2A as well as other HDAC proteins. The interacting complexes bind to specific regions of chromatin and regulate gene transcription in these regions.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic peptide surrounding amino acid 575 of human HDAC-5
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit anti-HDAC5 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blotting, Immunoprecipitation, Immunohistochemistry
Western blotting (0.5-4 µg/ml), Immunoprecipitation (20 µg/ml), and Immunohistochemistry (20 µg/ml). However, the optimal conditions should be determined individually. The antibody detects ~124 kDa HDAC5 with human and mouse samples, and in a lesser extent with rat samples. It does not cross-react with other HDAC proteins including HDAC1, 2, 3, 4, 6, 7, 8, 9,10, and 11.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.