Target of this antibody:
Alternative names of antibody target:
HD6, HDAC-6, histone deacetylase 6, JM21
Human HDAC6 (1215 a.a. residues) possesses two separate putative catalytic domains, both of which are fully functional and contribute independently to the overall activity of HDAC6. A very potent NES is present at the amino-terminus of HDAC6, which was found to play an important role in regulating the shuttling of HDAC6 protein between cytoplasm and nucleus. The shuttling process may be a critical regulatory mechanism of HDAC6 function. HDAC6 may participate in coordinating expression of a group of genes involved in the remoldeling of chromatin during cell differentiation.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic peptide mapping to the N-terminus of mouse HDAC-6
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.2 mg/ml) affinity purified rabbit anti-HDAC6 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blot, Immunohistochemistry
Western blotting (0.5-4 µg/ml) and Immunohistochemistry (20 µg/ml). However, the optimal conditions should be determined individually. The antibody detects ~134 kDa HDAC-6. Jurkat cell lysate can be used as a positive control. The antibody does not cross-react with other HDAC proteins including HDAC1, 2, 3, 4, 5, 7, and 8.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.