Target of this antibody:
Alternative names of antibody target:
MAP1 , MAP1A , MAP1L , MTAP1A , FLJ77111
Human MAP-1 (Modulator of Apoptosis Protein) is a novel Bax-associating protein. MAP-1 mediates caspase-dependent apoptosis in mammalian cells when overexpressed. MAP-1 homodimerizes and associates with the proapoptotic Bax and the prosurvival Bcl-2 and Bcl-XL of the Bcl-2 family.
Clonality of antibody:
This antibody reacts with:
Synthetic peptide surrounding a.a. 342 of mouse MAP-1 (
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) Protein A affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blotting (1-4 µg/ml). However, the optimal concentrations should be determined individually. Jurkat cell lysate (Cat.# 2401-100) and mouse small intestine tissue lysate (Cat.# 2601-100) can be used as positive controls. The antibody recognizes 39 kDa MAP-1 from samples of human, mouse and rat origins. Reactivity to other species has not been determined. In human samples, an additional ~78 kDa dimer can be detected in Jurkat cell lysate. In mouse sample, an additional ~33 kDa cleavage fragment can also be detected in mouse small intestine tissue lysate.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.