Target of this antibody:
Alternative names of antibody target:
NIF1, NIF-1, Nuclear Receptor Coactivator-Interacting Factor 1, ZNF335, Zinc Finger 335
NRC-Interacting Factor 1 (NIF1) interacts with the nuclear receptor transcriptional coactivator NRC and enhances ligand-dependent transcriptional activation by nuclear hormone receptors as well as c-Fos and c-Jun. NIF1 may function to influence chromatin structure.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic peptide surrounding amino acid 1324 of human NIF-1
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes over 100 kDa band corresponding to NIF-1 in samples from human origin. Reactivity to other species has not been tested.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.