Target of this antibody:
Alternative names of antibody target:
BRD-8, Bromodomain containing 8, SMAP, Skeletal Muscle Abundant Protein, Thyroid hormone receptor coactivating protein p120
BRD8 interacts with thyroid hormone receptor in a ligand-dependent manner and enhances thyroid hormone-dependent activation from thyroid response elements. BRD8 contains a bromodomain and is thought to be a nuclear receptor coactivator. Three alternatively spliced BRD8 transcript variants that encode distinct BRD8 isoforms have been identified.
Clonality of antibody:
This antibody reacts with:
Synthetic peptide of human BRD8
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µl affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 1% BSA, and 0.02% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blot, Immunoprecipitation
Western blotting (1:500 – 1:2000) and Immunoprecipitation. HeLa nuclear extract (Cat.# 1641-1) can be used as a positive control. However, the optimal concentrations should be determined individually. The antibody recognizes the BRD8 of human and mouse origins. Reactivity to other species has not been tested.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.