Target of this antibody:
Alternative names of antibody target:
E6AP, HPVE6AP, EPVE6AP, Human papillomavirus E6-associated protein; UBE3A, Ubiquitin-protein ligase E3A; AS, Angelman Syndrome; ANCR
E6AP is an E3 ubiquitin ligase. Inheritance of a mutant maternal copy of this gene results in Angelman syndrome (AS) which is characterized by severe mental retardation, seizures, ataxia, and EEG abnormalities. E6AP interacts with the type 16 and 18 human papillomavirus E6 oncoprotein which results in the targeting of p53 for degradation via the ubiquitin-proteasome system.
Clonality of antibody:
This antibody reacts with:
Synthetic peptide of human E6AP
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µl purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 1% BSA and 0.02% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blot, Immunoprecipitation
Western blotting (1:500 – 1:2500) and immunoprecipitation. However, the optimal concentrations should be determined individually. The antibody recognizes the E6AP of human and mouse origins. Reactivity to other species has not been tested
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.