Target of this antibody:
Alternative names of antibody target:
VCP, Valosin-Containing Protein, TERA, Transitional Endoplasmic Reticulum ATPase, p97, IBMPFD
Valosin containing protein (VCP), also designated TERA (for transitional endoplasmic reticulum ATPase) or p97, is a member of the AAA family of ATPases, which are involved in a variety of cellular activities. VCP is the mammalian homolog of Saccharomyces cerevisiae Cdc48, a protein essential for the completion of mitiosis in yeast. VCP is thought to be involved in a variety of membrane functions and in the regulation of the cell cycle. It associates with ubiquitinated IκB-αas well as with the 26S Proteosome, indicating a potential role for VCP in the proteosome-mediated degradation of IκB-α.
Clonality of antibody:
This antibody reacts with:
Synthetic peptide of human VCP
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µl affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 1% BSA, and 0.02% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blot, Immunoprecipitation
Western blotting (1:500 – 1:2000) and immunoprecipitation. However, the optimal concentrations should be determined individually. The antibody recognizes the 97 kDa VCP from samples of human and mouse origins. HeLa and NIH3T3 cell lysates can be used as positive controls. Reactivity to other species has not been tested.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.