anti-DKK2 Antibody

DKK2

DKK-2, DKK 2, dkk2, dkk-2, dkk 2, dickkopf homolog-2, dickkopf homolog2, dickkopf homolog 2

Xenopus Dickkopf (Dkk)-1 was initially discovered as a Wnt antagonist that plays an important role in head formation. By far, four members of Dkk have been identified in mammals. Each Dkk molecule contains two conserved cysteine-rich domains. Recent studies showed that the second Cys-rich domains of Dkk1 and Dkk2 inhibited Wnt-3a-activated signaling, whereas the first Cys-rich domains had no effects. In addition, the second Cys-rich domain of Dkk-2, but not that of Dkk-1, was able to activate the canonical pathway in the presence of LRP6, and this LRP-dependent signaling does not require Dvl.

Rabbit

Polyclonal

Rabbit IgG

Human, mouse, rat

Synthetic peptide surrounding amino acid 241 of mouse Dkk2

0.5 mg/ml

Liquid

100 µg (0.5 mg/ml) affinity purified rabbit Dkk2 polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.

Affinity purified

Western blot

Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes 57 kDa Dkk2 in samples from human, mouse and rat origins. A ~55 kDa and ~28 kDa Dkk2 fragments can also be detected in rat kidney tissue lysate. Reactivity to other species has not been determined.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps