Target of this antibody:
Alternative names of antibody target:
CNX, ER membrane marker
Calnexin (CNX) is a 90kDa integral protein of the endoplasmic reticulum (ER). Calnexin belongs among chaperones, which are characterized by their main function of assisting protein folding and quality control, ensuring that only properly folded and assembled proteins proceed further along the secretary pathway. The function of Calnexin is to retain unfolded or unassembled N-linked glycoproteins in the endoplasmic reticulum.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat, dog
Synthetic peptide surrounding amino acid 580 of human Calnexin
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes ~90-100 kDa band, corresponding to glycosylated Calnexin in samples from human, mouse, rat, and dog origins. Reactivity to other species has not been tested.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.