Target of this antibody:
Alternative names of antibody target:
BRK, FLJ42088 , PTK6 , breast tumor kinase
Breast tumor kinase, Brk (also known as PTK6), is a member of intracellular tyrosine kinase family that is thoμght to be related to Src family of tumor related kinases. Brk is expressed in breast carcinomas but not in normal breast tissue. The presence of Brk in the nucleus appears to be linked to suppression of tumor progression and has been shown to undergo autophosphorylation.
Clonality of antibody:
This antibody reacts with:
Synthetic peptide corresponding to residues surrounding amino acids 508 of human Brk
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit anti-Brk polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes ~57 kDa Brk of human, mouse, and rat origins. Reactivity to other species has not been tested.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.