Target of this antibody:
Alternative names of antibody target:
Sox2, Sox 2, SRY-box containing gene 2, sex determining region Y box-2, SRY related HMG box 2, Transcription factor SOX 2
Sox genes encode for transcriptional regulators that are involved in the early embryonic develpoment and in the determination of cell fate. Sox2 is also known as SRY (sex determining region Y)-box2, contain genes that encode for the HMG-box domain that is responsible for DNA binding activity. Mutation of Sox2 genes is link to optic nerve hypoplasia and anophthalmia, a severe form of eye deformity.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat, pig, bovine, dog, sheep
Synthetic peptide surrounding amino acid 301 of human Sox2
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) antigen affinity purified rabbit anti-Sox-2polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol and 0.01% Thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blotting (0.5-1 µg/ml). However, the optimal conditions should be determined individually. Other applications have not been tested. The antibody detects ~35kDa of Sox2 in samples from mouse origins.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.