Target of this antibody:
Alternative names of antibody target:
PRMT5, Protein Arginine Methyltransferase 5, HRMT1L5, HMT1 hnRNP Methyltransferase-Like 5, SKB1, Shk1 Kinase-Binding protein , IBP72, 72 kDa ICln-binding protein, JBP1, Jak-binding protein 1
Protein arginine N-methyltransferase 5 (PRMT-5) is a member of the PRMT family that localizes to the cytoplasm in many types of tissues. At least three types of PRMT enzymes have been identified that shows essential regulatory functions by methylation of key proteins.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic peptide surrounding amino acid 51 of rat PRMT-5
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit anti-PRMT-5 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal
This antibody has been succesfully tested for use in following methods:
Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Jurkat cell lysate can be used as a positive control.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.