Target of this antibody:
Alternative names of antibody target:
GELB , EC 126.96.36.199 , CLG4B , Matrix metalloproteinase
The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-9 has been shown to degrade bone collagens in concert with MMP-1 (also designated interstitial collagenase, fibroblast collagenase or collagenase-1), and cysteine proteases and may play a role in bone osteoclastic resorption.
Clonality of antibody:
This antibody reacts with:
E. coli-expressed recombinant human MMP-9
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
200 µg (0.5 mg/ml) affinity purified rabbit anti-human MMP-9 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5mM EDTA and 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
The antibody can be used in Western Blot analysis (1-4 µg/ml). However, the optimal conditions should be determined individually. Recombinant human MMP-9 (Cat.# 7789-10) can be used as a positive control.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.