Target of this antibody:
Since the molecular cloning of GFP cDNA and demonstration of GFP as a functional transgene, GFP has become a powerful tool with exciting applications in developmental, cell and molecular biology. YFP is the recent discovered protein that has the similar function and applications as GFP. Either GFP or YFP fluorescence is not species specific and can be expressed in bacteria, yeast, plant and mammalian cells. GFP or YFP can fuse with proteins of interest without interfering significantly with their assembly and function.
Clonality of antibody:
This antibody reacts with:
Recombinant YFP expressed from E. coli
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blotting (0.5-4 µg/ml), immunoprecipitation (10-20 µg/ml) and immunofluorescence. However, the optimal conditions should be determined individually.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.