anti-Sushi Antibody

Catalog number
anti-Sushi Antibody
Sushi Antibody
403.00 EUR


Target of this antibody:
Sushi 3
Alternative names of antibody target:
Factor C Sushi 3 antibody, Sushi 3 antibody
Background information:
Sushi Peptide S3 is a trimer of one of the high endotoxin-binding domains, Sushi 3 (or S3) within Factor C, a lipopolysaccharide (LPS)-sensitive serine protease of the horseshoe crab (Limulus Polyphemus). S3 display detergent-like properties in disrupting LPS aggregates, with specificity for palmitoyl-oleoyl-phosphatidylglycerol (POPG) resulting from electrostatic and hydrophobic forces between the peptides and the bacterial lipids. The unsaturated nature of POPG confers fluidity and enhances insertion of the peptides into the lipid bilayer, causing maximal disruption of the bacterial membrane. In short, peptide S3 can bind to lipopolysaccharide (LPS) and inhibit the growth of Gram-negative bacteria without affecting mammalian cells. It has been shown that endotoxin activates Factor C based catalytic coagulation cascade resulting in the gelation of Limulus blood. This process is the basis of Limulus Amebocyte Lysate (LAL) endotoxin detection method.
Antibody host:
Clonality of antibody:
Rabbit IgG
This antibody reacts with:
Immunogene sequence:
Sushi domain in from E. coli
Concentration of antibody:
0.5 mg/ml
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) polyclonal antibody in PBS pH 7.2, containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.
Purification method:
Affinity purified
This antibody has been succesfully tested for use in following methods:
Western Blot
Recommended usage:
Western Blot analysis (1-4 µg/ml).
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions:
gel pack
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation