anti-DRAM Antibody (NT)

DRAM

Damage-regulated autophagy modulator

Damage-regulated autophagy modulator (DRAM) is a p53 target gene encoding a lysosomal protein that induces autophagy, a process that degrades cytosolic proteins and organelles. It has been sμggested that activation of DRAM by p53 is simultaneous to the activation by p53 of one or more proapoptotic genes such as PUMA, Bax, etc., and that the signaling pathways regulated by these genes promote a full cell death response. By itself, DRAM cannot induce apoptosis, but the fact that it is inactivated in certain cancers highlights the importance of DRAM and sμggests that autophagy may play a more important role in cancer than initially suspected. At least two different isoforms of DRAM are known to exist.

Rabbit

Polyclonal

Rabbit IgG1

Human, mouse, rat

DRAM antibody was raised against a 16 amino acid peptide from near the amino terminus of human DRAM.

1 mg/ml

Liquid

100 µg (1 mg/ml) in 1X PBS containing 0.02% sodium azide.

Affinity chromatography purified via peptide column.

Western blot, Immunohistochemistry, ELISA

Western Blot: 0.5-2 μg/ml, Immunohistochemistry: 2.5 μg/ml, ELISA. However, the optimal conditions should be determined individually.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps